"Beer-Lambert Law Explained: The Power Behind UV-Vis Analysis"
International Chemistry Scientist Awards
UV-Vis Spectroscopy (Ultraviolet-Visible Spectroscopy) is a widely used analytical technique in chemistry and biochemistry for analyzing the absorbance and transmittance of light through a sample. This technique helps identify and quantify compounds based on their electronic transitions, particularly those involving π to π* or n to π* transitions. UV-Vis spectroscopy plays a crucial role in monitoring reaction progress, determining concentration via Beer-Lambert’s law, and analyzing structural features of organic and inorganic molecules. Its simplicity, speed, and non-destructive nature make it an essential tool across research, pharmaceutical, environmental, and industrial laboratories.
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